Hadjod is a perennial plant of
the grape family and found all over India. It is one of the best herb
known for regeneration of bones and cartilages. It directly nourishes to
bone tissues and also strengthens cartilages and ligaments. It helps in
reunion of bone that is why it shows good result in bone fracture and
activities of the petroleum ether extract of Cissus quadrangularis Linn.
in ovariectomized Wistar rats.
The plant Cissus quadrangularis (CQ) is
used as an osteoprotective agent in Ayurveda, the Indian system of
alternative medicine. The present study was done to validate the anti-osteoporotic
role of the petroleum ether extract of CQ on ovariectomy-induced
osteoporosis in rats.
METHODS: Female wistar rats were divided
into five groups of six rats each; (1) The Normal control (NC) group (n=6)
received no surgery and no treatment, (2). The Sham control (SHAM) group
(n=6) received sham surgery but no treatment, (3) the Ovariectomized (OVX)
group (n=6) received an ovariectomy and normal saline treatment for 90
days, (4) the Ovariectomized+raloxifene (OVX+RAL) group (n=6) received an
ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent
for 90 days commencing from 22nd post ovariectomy day and (5) the
Ovariectomy+Cissus quadrangularis (OVX+CQ) group (n=6) received an
ovariectomy, and treatmentwith the petroleum ether extract of CQ 500 mg/kg
body weight daily for 90 days commencing from 22nd post ovariectomy day.
At the end of the treatment period, rats in all groups were sacrificed and
the right femur was used for biomechanical analysis, and the left femur
for histomorphometrical analysis. RESULTS: CQ significantly increased the
force required to break the femur (p<0.001) and significantly increased
the thickness of both cortical (p<0.001) and trabecular bone (p<0.001).
This action of CQ was comparable to the action of raloxifene. CONCLUSIONS:
The petroleum ether extract of CQ stem seems to possess anti-osteoporotic
activity in rats.